TSRI's main web site PROMISE mirror at TSRI Metalloprotein DB site Created: 7 November 1996
Last modified: 11 August 1997

P450cam structure [1, 2]

Click on image to get the corresponding picture in PostScript format. Be aware of the size of the files:
a = 257 K; b = 254 K; c = 14 K; d = 15 K; e = 12 K; f = 13 K.

PDB scop BSM RELI
Base		 Header
1phc 1phc 1phc 1phc P450cam; Pseudomonas putida
3cpp 3cpp 3cpp 3cpp P450cam (complex with CO and reduced camphor); Pseudomonas putida
3CPP image A
a		 3CPP image B
b
3CPP active site image A

c

 3CPP active site image B

d
1PHC active site image A

e

 1PHC active site image B

f

  1. Ribbon representation of the Pseudomonas putida P450cam complex with CO and camphor structure (PDB code 3CPP) [1].
  2. As a, rotated 90° around y axis.
  3. Active site structure: haem, bound CO and camphor, Phe­87, Tyr­96 and Thr­253.
  4. As c, plus Cys­357 (haem iron proximal ligand); rotated 90° around y axis.
  5. Active site structure of substrate­free P450cam (PDB code 1PHC) [2].
  6. As e, plus Cys­357; rotated 90° around y axis.
All drawings were produced by using the program MOLSCRIPT [3].

References

  1. Raag, R. and Poulos, T.L. (1989) Crystal structure of the carbon monoxide-substrate-cytochrome P­450CAM ternary complex. Biochemistry 28, 7586-7592.
  2. Poulos, T.L., Finzel, B.C. and Howard, A.J. (1986) Crystal structure of substrate­free Pseudomonas putida cytochrome P­450. Biochemistry 25, 5314-5322.
  3. Kraulis, P.J. (1991) MOLSCRIPT: a program to produce both detailed and schematic plots of protein structure. J. Appl. Crystallogr. 24, 946-950.
Bibliography on structural studies of P450s