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Last modified: 22 May 1997

2,3­Dihydroxybiphenyl 1,2­dioxygenase structure [1]

Click on image to get the corresponding picture in PostScript format. Be aware of the size of the files:
a = 190 K; b = 64 K.

PDB scop BSM RELI
Base		 Header MMS Abstract ¹
1han 1han 1han 1han 2,3­Dihydroxybiphenyl 1,2­dioxygenase (BhpC enzyme) (complex with tertiary­butyl alcohol); Pseudomonas cepacia strain LB400 MS6TG1
1HAN image
a		 1HAN Fe centre image
b

¹ Macromolecular Structures abstract. Full text is available to BioMedNet Members

  1. Ribbon representation of the Pseudomonas cepacia 2,3­dihydroxybiphenyl 1,2­dioxygenase (PDB code 1HAN) complex with t­butanol. Domain A (2-118) = blue; domain B (146-289) = green. Note that the protein binds two iron atoms: an active site iron buried in the domain B and an adventitious iron present with partial occupancy and is derived from the crystal stabilising solution.
  2. Mononuclear iron centre and its ligands.
All drawings were produced by using the program MOLSCRIPT [2].

References

  1. Han, S., Eltis, L.D., Timmis, K.N., Muchmore, S.W. and Bolin, J.T. (1995) Crystal structure of the biphenyl­cleaving extradiol dioxygenase from a PCB­degrading pseudomonad. Science 270, 976-980.
  2. Kraulis, P.J. (1991) MOLSCRIPT: a program to produce both detailed and schematic plots of protein structure. J. Appl. Crystallogr. 24, 946-950.
Bibliography on structural studies of extradiol aromatic­ring­cleavage dioxygenases